Hemoglobin Brockton [beta 138 (H16) Ala—-Professional] has lower oxygen affinity but electrophoretic mobility and hemolysate binding are typical, suggesting the substitution at beta 138 would not disrupt significant polar interactions, like the hydrogen bonds and salt bridges concerning Asp 137 and Pro 138, that add to the cooperative oxygen-binding process https://nicolaslecourtmansion.com