Novel leafy seadragon (Phycodurus eques) microsatellite loci were developed via standard cloning techniques and tested for use in population genetics studies. Six out of a total of twelve microsatellites tested were usable for population analysis. Seadragon samples from Western Australia (N = 6). Southern Australia (N = 11). https://chefesquipmenters.shop/product-category/cooling-racks-grates/